Cyclooxygenase-2 expression is up-regulated in squamous cell carcinoma of the head and neck.

نویسندگان

  • G Chan
  • J O Boyle
  • E K Yang
  • F Zhang
  • P G Sacks
  • J P Shah
  • D Edelstein
  • R A Soslow
  • A T Koki
  • B M Woerner
  • J L Masferrer
  • A J Dannenberg
چکیده

The purpose of this study was to determine whether cyclooxygenase-2 (COX-2) was overexpressed in squamous cell carcinoma of the head and neck (HNSCC). Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in head and neck tissue. Mean levels of COX-2 mRNA were increased by nearly 150-fold in HNSCC (n = 24) compared with normal oral mucosa from healthy volunteers (n = 17). Additionally, there was about a 50-fold increase in amounts of COX-2 mRNA in normal-appearing epithelium adjacent to HNSCC (n = 10) compared with normal oral mucosa from healthy volunteers. Immunoblotting demonstrated that COX-2 protein was present in six of six cases of HNSCC but was undetectable in normal oral mucosa from healthy subjects. Immunohistochemical analysis showed that COX-2 was expressed in both HNSCC and adjacent normal-appearing epithelium. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of HNSCC.

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عنوان ژورنال:
  • Cancer research

دوره 59 5  شماره 

صفحات  -

تاریخ انتشار 1999